Our Story
My name is Denise and my husband's name is Scot. Four years ago, we met in Rio de Janeiro, where I am from originally. Since my husband has cystic fibrosis, we knew it would be difficult for us to have any children naturally, if we were to get married. Despite this, we were married three and a half years ago. When it came the time to make a decision about having a baby, we struggled with the choice of trying IVF or adoption. Initially, our decision was the adoption route, but at the last minute, I had a change of heart. I told my husband I really thought we should try IVF or else we would never know if we would have been able to have a child of our own. At the time, I was 37 years old, adding to our difficulties. Once we committed to trying IVF, we met with Dr. Silber and his staff to begin the process. We endured the whole process of the medication regime, injection schedules, and the anxiety that accompanies it all.
When our retrieval date arrived, not only were we very nervous, but we ran into more problems. Dr. Silber discovered my husband had a congenital defect, making the sperm retrieval difficult. After some searching, he was able to retrieve two sperm from my husband. From me, they were able to retrieve only two eggs. After fertilization, we learned that we only had one viable embryo to implant. Despite our low odds, I never gave up hope.
My mom in Brazil would keep telling me that everything was going well and she knew for certain that I would get pregnant. I truly believed in what she told me. I can not describe the feeling of joy I had when I was told by Dena that I was pregnant. Even now, writing our story brings many, many tears to my eyes. It is a blessing to be able to look at our beautiful, healthy daughter Giovanna everyday and I encourage anyone to try because it is the most wonderful feeling one can have. We are so grateful to Dr. Silber and his staff for helping us start our family.
Denise, Scot and Giovanna Colgrove
Fenton, Missouri
In vitro fertilization (IVF) is a process by which egg cells are fertilised by sperm outside the body, in vitro. IVF is a major treatment in infertility when other methods of assisted reproductive technology have failed. The process involves hormonally controlling the ovulatory process, removing ova (eggs) from the woman's ovaries and letting sperm fertilise them in a fluid medium. For More Detailed Information : IVF matters
Monday, 23 May 2011
Patient Comments - 17
Dear Dr. Silber,
My husband and I were married in 2000. In 2003, we decided we would like to try having children, however, Mark had undergone a vasectomy about 17 years prior. After researching reversal options on the internet, I happened upon Dr. Silber's office and was very impressed by his success rate, even with "old" vasectomies. In February 2004, Mark underwent a reversal... and endured a couple of weeks of teasing while he walked slightly bowlegged! Six months later we became pregnant! Our daughter was born healthy and happy, and has since been joined by a lively younger sister. We HIGHLY recommend Dr. Silber for his excellent work, professionalism and kindness. It was worth the travel and every penny.
With happy regards from Wisconsin,
Mark and Nancy Tritt
Amery, WI
My husband and I were married in 2000. In 2003, we decided we would like to try having children, however, Mark had undergone a vasectomy about 17 years prior. After researching reversal options on the internet, I happened upon Dr. Silber's office and was very impressed by his success rate, even with "old" vasectomies. In February 2004, Mark underwent a reversal... and endured a couple of weeks of teasing while he walked slightly bowlegged! Six months later we became pregnant! Our daughter was born healthy and happy, and has since been joined by a lively younger sister. We HIGHLY recommend Dr. Silber for his excellent work, professionalism and kindness. It was worth the travel and every penny.
With happy regards from Wisconsin,
Mark and Nancy Tritt
Amery, WI
The History of IVF -The Milestones
1998
First establish pregnancy using recombinant FSH and GnRH antagonist (Itskovitz-Eldor J, Kol S, Mannaerts B, Coelingh Bennink H. First established pregnancy after controlled ovarian hyperstimulation with recombinant follicle stimulating hormone and the gonadotrophin-releasing hormone antagonist ganirelix (Org 37462). Hum Reprod. 1998;13:294-5).
Gardner introduced sequential media and blastocyst transfer which is now greatly assists in the move to single embryo transfer (Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 1998;13:3434-40).
Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter's syndrome (Palermo GD, Schlegel PN, Sills ES, Veeck LL, Zaninovic N, Menendez S, Rosenwaks Z. Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter's syndrome. N Engl J Med. 1998, 26;338(9):588-90).
The first pregnancies following embryo culture in sequential media and transfer at the blastocyst stage of development (Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C. Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod. 1998 ;13:169-77).
First ovum pick-up under 3-D ultrasound vision (Feichtinger W. Follicle aspiration with interactive three-dimensional digital imaging (Voluson): a step toward real-time puncturing under three-dimensional ultrasound control. Fertil Steril. 1998;70(2):374-7).
In 1998 first reports on births from frozen GV eggs, and donor eggs: - Tucker et al. Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. (Tucker MJ, Wright G, Morton PC, Massey JB. Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil Steril. 1998;70(3):578-9); Tucker et al. Clinical application of human egg cryopreservation. (Tucker MJ, Morton PC, Wright G, Sweitzer CL, Massey JB. Clinical application of human egg cryopreservation. Hum Reprod. 1998;13(11):3156-9).
First establish pregnancy using recombinant FSH and GnRH antagonist (Itskovitz-Eldor J, Kol S, Mannaerts B, Coelingh Bennink H. First established pregnancy after controlled ovarian hyperstimulation with recombinant follicle stimulating hormone and the gonadotrophin-releasing hormone antagonist ganirelix (Org 37462). Hum Reprod. 1998;13:294-5).
Gardner introduced sequential media and blastocyst transfer which is now greatly assists in the move to single embryo transfer (Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 1998;13:3434-40).
Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter's syndrome (Palermo GD, Schlegel PN, Sills ES, Veeck LL, Zaninovic N, Menendez S, Rosenwaks Z. Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter's syndrome. N Engl J Med. 1998, 26;338(9):588-90).
The first pregnancies following embryo culture in sequential media and transfer at the blastocyst stage of development (Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C. Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod. 1998 ;13:169-77).
First ovum pick-up under 3-D ultrasound vision (Feichtinger W. Follicle aspiration with interactive three-dimensional digital imaging (Voluson): a step toward real-time puncturing under three-dimensional ultrasound control. Fertil Steril. 1998;70(2):374-7).
In 1998 first reports on births from frozen GV eggs, and donor eggs: - Tucker et al. Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. (Tucker MJ, Wright G, Morton PC, Massey JB. Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil Steril. 1998;70(3):578-9); Tucker et al. Clinical application of human egg cryopreservation. (Tucker MJ, Morton PC, Wright G, Sweitzer CL, Massey JB. Clinical application of human egg cryopreservation. Hum Reprod. 1998;13(11):3156-9).
The History of IVF -The Milestones
1997
First report on cytoplasmic transfer (Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet, 1997, 19;350(9072):186-7)
Sun, Jurisicova and Casper described the use of TUNEL for detection of DNA fragmentation in sperm and correlation with IVF outcome. They showed almost uniform presence of DNA fragmentation in round spermatids as the explanation for failure to achieve pregnancy with these immature gametes. (Sun JG, Jurisicova A, Caspe RF. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997 Mar;56(3):602-7).
First births of babies from frozen oocytes following use of ICSI: - Porcu et al, Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. (Porcu E, Fabbri R, Seracchioli R, Ciotti PM, Magrini O, Flamigni C. Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril. 1997;68(4):724-6).
First report on cytoplasmic transfer (Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet, 1997;19;350:186-7).
Falloposcopic GIFT: Births after transcervical gamete intrafallopian transfer with a falloposcopic delivery system. (Porcu E, Dal Prato L, Seracchioli R, Petracchi S, Fabbri R, Flamigni C. Births after transcervical gamete intrafallopian transfer with a falloposcopic delivery system. Fertil Steril. 1997 Jun;67(6):1175-7).
Pregnancy after treatment with three recombinant gonadotropins (Agrawal R, West C, Conway GS, Page ML, Jacobs HS. Pregnancy after treatment with three recombinant gonadotropins. Lancet. 1997;4;349:29-30).
First report on cytoplasmic transfer (Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet, 1997, 19;350(9072):186-7)
Sun, Jurisicova and Casper described the use of TUNEL for detection of DNA fragmentation in sperm and correlation with IVF outcome. They showed almost uniform presence of DNA fragmentation in round spermatids as the explanation for failure to achieve pregnancy with these immature gametes. (Sun JG, Jurisicova A, Caspe RF. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997 Mar;56(3):602-7).
First births of babies from frozen oocytes following use of ICSI: - Porcu et al, Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. (Porcu E, Fabbri R, Seracchioli R, Ciotti PM, Magrini O, Flamigni C. Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril. 1997;68(4):724-6).
First report on cytoplasmic transfer (Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet, 1997;19;350:186-7).
Falloposcopic GIFT: Births after transcervical gamete intrafallopian transfer with a falloposcopic delivery system. (Porcu E, Dal Prato L, Seracchioli R, Petracchi S, Fabbri R, Flamigni C. Births after transcervical gamete intrafallopian transfer with a falloposcopic delivery system. Fertil Steril. 1997 Jun;67(6):1175-7).
Pregnancy after treatment with three recombinant gonadotropins (Agrawal R, West C, Conway GS, Page ML, Jacobs HS. Pregnancy after treatment with three recombinant gonadotropins. Lancet. 1997;4;349:29-30).
The History of IVF -The Milestones
1996
The Valencia group reported on the first pregnancy employing cryopreserved testicular sperm following IVF-ICSI. . (Gil-Salom M, Romero J, Minguez Y, Rubio C, De los Santos MJ, Remohà J, Pellicer A. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod. 1996;11(6):1309-13).
Discovery that some men with severe oligoasthenospermia have deletions in the Y-chromosome (Reijo R, Alagappan RK, Patrizio P, Page DC. Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome. Lancet, 1996, 11;347(9011):1290-3).
Jurisicova from the Casper group was the first to recognize that preimplantation of human embryo fragmentation involved programmed cell death. (Jurisicova A, Varmuza S, Casper RF. Programmed cell death and human embryo fragmentation. Mol Hum Reprod. 1996;2(2):93-8).
Casper and his colleagues were the first to demonstrate and introduce the use of the hypo-osmotic swelling test for selection of immotile sperm for ICSI. (Casper et al. 1996 Fertil Steril 65:972)
The Valencia group reported on the first pregnancy employing cryopreserved testicular sperm following IVF-ICSI. . (Gil-Salom M, Romero J, Minguez Y, Rubio C, De los Santos MJ, Remohà J, Pellicer A. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod. 1996;11(6):1309-13).
Discovery that some men with severe oligoasthenospermia have deletions in the Y-chromosome (Reijo R, Alagappan RK, Patrizio P, Page DC. Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome. Lancet, 1996, 11;347(9011):1290-3).
Jurisicova from the Casper group was the first to recognize that preimplantation of human embryo fragmentation involved programmed cell death. (Jurisicova A, Varmuza S, Casper RF. Programmed cell death and human embryo fragmentation. Mol Hum Reprod. 1996;2(2):93-8).
Casper and his colleagues were the first to demonstrate and introduce the use of the hypo-osmotic swelling test for selection of immotile sperm for ICSI. (Casper et al. 1996 Fertil Steril 65:972)
Sunday, 22 May 2011
Patient Comments - 16
My husband and I had three children and then decided to get a vasectomy. A few years later we were led by the Lord to leave our fertility in His hands. We decided to go to the best, Dr. Silber. We wanted expert care for my husband. After surgery Dr. Silber talked with me and even shared pictures. It was amazing. My husband opted out of seeing the pictures! Anyway, we conceived 6 mos after the surgery with our first reversal miracle and had a boy in April of 2007, one year and two months after the surgery. We conceived again 9 mos later and had another boy in Sept. 2008. We conceived again two years later and are due Sept. of 2010. I am 40 and my husband is 44 and we had these babies 7 years after the original vasectomy.
Dr. Silber's office staff is amazing too and I always felt I could call with questions and they celebrated with me every time I called to announce another pregnancy. I wouldn't hesitate to recommend this to anyone considering it. It was through the Lord and Dr. Silber's hands that we have three new lives into this world that wouldn't have been here without a reversal. Our lives are blessed.
William and Wendy K.
St. Louis, MO
Dr. Silber's office staff is amazing too and I always felt I could call with questions and they celebrated with me every time I called to announce another pregnancy. I wouldn't hesitate to recommend this to anyone considering it. It was through the Lord and Dr. Silber's hands that we have three new lives into this world that wouldn't have been here without a reversal. Our lives are blessed.
William and Wendy K.
St. Louis, MO
Patient Comments - 15
We were first introduced to Dr. Silber by a friend of Ashley's who was an employee of Dr. Silber’s. From the very first appointment with Dr. Silber, we were very impressed with him and his staff. We were treated as a high priority with care and respect. Through a second round of IVF we conceived a beautiful little blessing known as Georgia Lou Powell. Without the Lord, Dr. Silber, and his amazing staff we would not have our beautiful baby girl.
Jarrod, Ashley, and Georgia Powell
Moscow Mills, MO
Jarrod, Ashley, and Georgia Powell
Moscow Mills, MO
The History of IVF -The Milestones
1995
Pregnancies after Testicular Sperm Extraction (TESE) and ICSI in non-obstructive azoospermia. (Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, Van Steirteghem A, Silber S. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10(6):1457-60).
Birth after blastocyst development from IVM oocyte plus ICSI plus Assisted Hatching (Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10(12):3243-7).
The first report of aneuploidy testing (Munné S, Sultan KM, Weier HU, Grifo JA, Cohen J, Rosenwaks Z. Assessment of numeric abnormalities of X, Y, 18, and 16 chromosomes in preimplantation human embryos before transfer. Am J Obstet Gynecol. 1995;172(4 Pt 1):1191-9; discussion 1199-201 ).
Zenzes from the group of Casper in Toronto demonstrated adverse effects of cigarette smoking on nuclear spindle. (Zenzes MT, Wang P, Casper RF. Cigarette smoking may affect meiotic maturation of human oocytes. Hum Reprod. 1995;10(12):3213-17).
The first report in which spermatids were used to achieve pregnancy. (Fishel S, Green S, Bishop M, Thornton S, Hunter A, Fleming S, al-Hassan S. Pregnancy after intracytoplasmic injection of spermatid. Lancet. 1995, 24;345(8965):1641-2).
Dmitri Dozortsev and coworkers have discovered that oocyte activation during ICSI is triggered by a water-soluble, heat-sensitive, non species specific cytosolic sperm factor. (Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M. Human oocyte activation following intracytoplasmic injection: the role of the sperm cell. Hum Reprod. 1995;10(2):403-7).
The larget study done in the filed of IVF comparing urinary FSH with Rec-FSH (Out, H.J., Mannaerts, B.M., Driessen, S.G. and Coelingh Bennink, H.J. A prospective, randomized, assessor blind, multicenter study comparing recombinant and urinary follicle-stimulating hormone in in vitro fertilization. Hum. Reprod., 1995: 10, 2534 –2540).
Pregnancies after Testicular Sperm Extraction (TESE) and ICSI in non-obstructive azoospermia. (Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, Van Steirteghem A, Silber S. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10(6):1457-60).
Birth after blastocyst development from IVM oocyte plus ICSI plus Assisted Hatching (Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10(12):3243-7).
The first report of aneuploidy testing (Munné S, Sultan KM, Weier HU, Grifo JA, Cohen J, Rosenwaks Z. Assessment of numeric abnormalities of X, Y, 18, and 16 chromosomes in preimplantation human embryos before transfer. Am J Obstet Gynecol. 1995;172(4 Pt 1):1191-9; discussion 1199-201 ).
Zenzes from the group of Casper in Toronto demonstrated adverse effects of cigarette smoking on nuclear spindle. (Zenzes MT, Wang P, Casper RF. Cigarette smoking may affect meiotic maturation of human oocytes. Hum Reprod. 1995;10(12):3213-17).
The first report in which spermatids were used to achieve pregnancy. (Fishel S, Green S, Bishop M, Thornton S, Hunter A, Fleming S, al-Hassan S. Pregnancy after intracytoplasmic injection of spermatid. Lancet. 1995, 24;345(8965):1641-2).
Dmitri Dozortsev and coworkers have discovered that oocyte activation during ICSI is triggered by a water-soluble, heat-sensitive, non species specific cytosolic sperm factor. (Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M. Human oocyte activation following intracytoplasmic injection: the role of the sperm cell. Hum Reprod. 1995;10(2):403-7).
The larget study done in the filed of IVF comparing urinary FSH with Rec-FSH (Out, H.J., Mannaerts, B.M., Driessen, S.G. and Coelingh Bennink, H.J. A prospective, randomized, assessor blind, multicenter study comparing recombinant and urinary follicle-stimulating hormone in in vitro fertilization. Hum. Reprod., 1995: 10, 2534 –2540).
The History of IVF -The Milestones
1994
The first live birth as a result of IVM following transvaginal ultrasound–guided oocyte collection. (Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62(2):353-62).
Development of the first highly purified FSH preparation for use in IVF (Howles CM, Loumaye E, Giroud D, Luyet G. Multiple follicular development and ovarian steroidogenesis following subcutaneous administration of a highly purified urinary FSH preparation in pituitary desensitized women undergoing IVF: a multicentre European phase III study. Hum Reprod. 1994;9(3):424-30). First pregnancy after use of r-hLH (Hull M, Corrigan E, Piazzi A, Loumaye E. Recombinant human luteinising hormone: an effective new gonadotropin preparation. Lancet 1994;30;344(8918):334-5).
The first live birth as a result of IVM following transvaginal ultrasound–guided oocyte collection. (Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62(2):353-62).
Development of the first highly purified FSH preparation for use in IVF (Howles CM, Loumaye E, Giroud D, Luyet G. Multiple follicular development and ovarian steroidogenesis following subcutaneous administration of a highly purified urinary FSH preparation in pituitary desensitized women undergoing IVF: a multicentre European phase III study. Hum Reprod. 1994;9(3):424-30). First pregnancy after use of r-hLH (Hull M, Corrigan E, Piazzi A, Loumaye E. Recombinant human luteinising hormone: an effective new gonadotropin preparation. Lancet 1994;30;344(8918):334-5).
The History of IVF -The Milestones
1993
The second term pregnancy after ICSI reported by a group in Sweden (Hamberger et al, 1993, In Gordts S (ed) Proceedings of European Symposium on Micromanipulation. Drukkerij Nauwelaerts, Leuven, Belgium, pp 85).
Confirmation that men with congenital absence of the vas defference, have cystic fibrosis mutations which can be transmitted to the offspring (Patrizio P, Asch RH, Handelin B, Silber SJ. Aetiology of congenital absence of vas deferens: genetic study of three generations. Hum Reprod. 1993;8(2):215-20).
First report on the use of TESE (testicular sperm extraction) and ICSI (Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod. 1994;9(9):1705-9).
First Preganancy and birth following treatment with Rec-FSH (Devroey P, Mannaerts B, Smith J, Coelingh Bennink H, Van Steirteghem A. First established pregnancy and birth after ovarian stimulation with recombinant human follicle stimulating hormone (Org 32489). Hum Reprod.1993 Jun;8(6):863-5).
The second term pregnancy after ICSI reported by a group in Sweden (Hamberger et al, 1993, In Gordts S (ed) Proceedings of European Symposium on Micromanipulation. Drukkerij Nauwelaerts, Leuven, Belgium, pp 85).
Confirmation that men with congenital absence of the vas defference, have cystic fibrosis mutations which can be transmitted to the offspring (Patrizio P, Asch RH, Handelin B, Silber SJ. Aetiology of congenital absence of vas deferens: genetic study of three generations. Hum Reprod. 1993;8(2):215-20).
First report on the use of TESE (testicular sperm extraction) and ICSI (Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod. 1994;9(9):1705-9).
First Preganancy and birth following treatment with Rec-FSH (Devroey P, Mannaerts B, Smith J, Coelingh Bennink H, Van Steirteghem A. First established pregnancy and birth after ovarian stimulation with recombinant human follicle stimulating hormone (Org 32489). Hum Reprod.1993 Jun;8(6):863-5).
Friday, 20 May 2011
Pateints comments - 14
Dr. Silber,
I just wanted to drop you a note thanking you for your part in the birth of our son – Hugh Benjamin Jay. He was born November 3rd and weighed 6 lbs. 13 oz. May God Bless you for all that you do. We are eternally grateful.
Love,
Ben & Chanda Ruff
Bay City, MI
I just wanted to drop you a note thanking you for your part in the birth of our son – Hugh Benjamin Jay. He was born November 3rd and weighed 6 lbs. 13 oz. May God Bless you for all that you do. We are eternally grateful.
Love,
Ben & Chanda Ruff
Bay City, MI
Pateints comments - 13
Dear Dr. Silber, Dr. DeRosa and staff,
We are writing to inform you of the birth of our fraternal twin sons, Abram David and Colton Willard. They are two beautiful, healthy, happy boys. They were born on August 8 at 37 weeks and 2 days via vaginal delivery without any complications and came home with us on the 10th. They are doing wonderful and are growing and changing every day. They already have their own individual personalities. Abram is laid back, happy and smiling all the time and Colton is a character who likes to cuddle and be held all the time. We feel so blessed to be writing this letter and we know it would not be possible without you. Everyone was such a pleasure to work with, especially Dena, our IVF consultant who we received the good news from! We cannot thank you all enough… you have helped make our dream come true of having a family and we will never forget you.
God Bless,
David & Emily Seaton
Collinsville, IL
We are writing to inform you of the birth of our fraternal twin sons, Abram David and Colton Willard. They are two beautiful, healthy, happy boys. They were born on August 8 at 37 weeks and 2 days via vaginal delivery without any complications and came home with us on the 10th. They are doing wonderful and are growing and changing every day. They already have their own individual personalities. Abram is laid back, happy and smiling all the time and Colton is a character who likes to cuddle and be held all the time. We feel so blessed to be writing this letter and we know it would not be possible without you. Everyone was such a pleasure to work with, especially Dena, our IVF consultant who we received the good news from! We cannot thank you all enough… you have helped make our dream come true of having a family and we will never forget you.
God Bless,
David & Emily Seaton
Collinsville, IL
The History of IVF -The Milestones
1992
Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Presented simultaneously by two groups: (Germond M, Dessole S, Senn A, Loumaye E, Howles C, Beltrami V. Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Lancet, 1992 9;339:1170) (Devroey P, van Steirteghem A, Mannaerts B, Bennink HC. Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Lancet. 1992;9;339:1170).
Assisted zona hatching was introduced in IVF programs to breach the zona pellucida and promote the natural process of hatching. (Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum. Reprod. 1992 7: 685-691).
Discovery that men with congenital absence of the vas deferens have a mild form of cystic fibrosis: Two papers:
(Anguiano et al. 1992, JAMA 267(13)1794)(Patrizio and Asch 1992, Annals Acad Med Singapore 21:533)
Report of the first pregnancy after intracytoplasmic sperm injection (ICSI) by the group in Brussels (Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;4;340:17-8).
Pregnancy after embryo biopsy and coamplification of DNA from X and Y chromosomes. (Grifo JA, Tang YX, Cohen J, Gilbert F, Sanyal MK, Rosenwaks Z. Pregnancy after embryo biopsy and coamplification of DNA from X and Y chromosomes. JAMA 1992, 12;268(6):727-9).
Ovulation induction by endogenous LH released by the administration of an LHRH agonist after follicular stimulation for in vitro fertilization (Emperaire JC, Ruffié A, Audebert AJ. Ovulation induction by endogenous LH released by the administration of an LHRH agonist after follicular stimulation for in vitro fertilization. J Gynecol Obstet Biol Reprod (Paris). 1992;21:489-94. French).
First report on cumulative conception and live birth rates after IVF in relation to patient's age and cause of infertility (Tan SL, Royston P, Campbell S, Jacobs HS, Betts J, Mason B, Edwards RG. Cumulative conception and livebirth rates after in-vitro fertilisation. Lancet. 1992,6;339(8806):1390-4).
First two births from replacement of frozen embryos produced with epididymal sperm (Patrizio P, Silber S, Ord T, Marello E, Balmaceda JP, Asch RH. Replacement of frozen embryos generated from epididymal spermatozoa: the first two pregnancies. Hum Reprod 1992;7(5):652-3)
Report on using Erbium YAG laser for micromanipulation of oocytes and spermatozoa (Feichtinger W, Strohmer H, Radner KM. Erbium YAG laser for micromanipulation of oocytes and spermatozoa. Lancet. 1992, 11;340(8811):115-6).
Investigating the role of FSH in hypogonadotropic women usung the new recombinant FSH drug (Schoot DC, Coelingh Bennink HJ, Mannaerts BM, Lamberts SW, Bouchard P, Fauser BC. Human recombinant follicle-stimulating hormone induces growth of preovulatory follicles without concomitant increase in androgen and estrogen biosynthesis in a woman with isolated gonadotropin deficiency. J Clin Endocrinol Metab 1992; 74: 1471-3).
Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Presented simultaneously by two groups: (Germond M, Dessole S, Senn A, Loumaye E, Howles C, Beltrami V. Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Lancet, 1992 9;339:1170) (Devroey P, van Steirteghem A, Mannaerts B, Bennink HC. Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Lancet. 1992;9;339:1170).
Assisted zona hatching was introduced in IVF programs to breach the zona pellucida and promote the natural process of hatching. (Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum. Reprod. 1992 7: 685-691).
Discovery that men with congenital absence of the vas deferens have a mild form of cystic fibrosis: Two papers:
(Anguiano et al. 1992, JAMA 267(13)1794)(Patrizio and Asch 1992, Annals Acad Med Singapore 21:533)
Report of the first pregnancy after intracytoplasmic sperm injection (ICSI) by the group in Brussels (Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;4;340:17-8).
Pregnancy after embryo biopsy and coamplification of DNA from X and Y chromosomes. (Grifo JA, Tang YX, Cohen J, Gilbert F, Sanyal MK, Rosenwaks Z. Pregnancy after embryo biopsy and coamplification of DNA from X and Y chromosomes. JAMA 1992, 12;268(6):727-9).
Ovulation induction by endogenous LH released by the administration of an LHRH agonist after follicular stimulation for in vitro fertilization (Emperaire JC, Ruffié A, Audebert AJ. Ovulation induction by endogenous LH released by the administration of an LHRH agonist after follicular stimulation for in vitro fertilization. J Gynecol Obstet Biol Reprod (Paris). 1992;21:489-94. French).
First report on cumulative conception and live birth rates after IVF in relation to patient's age and cause of infertility (Tan SL, Royston P, Campbell S, Jacobs HS, Betts J, Mason B, Edwards RG. Cumulative conception and livebirth rates after in-vitro fertilisation. Lancet. 1992,6;339(8806):1390-4).
First two births from replacement of frozen embryos produced with epididymal sperm (Patrizio P, Silber S, Ord T, Marello E, Balmaceda JP, Asch RH. Replacement of frozen embryos generated from epididymal spermatozoa: the first two pregnancies. Hum Reprod 1992;7(5):652-3)
Report on using Erbium YAG laser for micromanipulation of oocytes and spermatozoa (Feichtinger W, Strohmer H, Radner KM. Erbium YAG laser for micromanipulation of oocytes and spermatozoa. Lancet. 1992, 11;340(8811):115-6).
Investigating the role of FSH in hypogonadotropic women usung the new recombinant FSH drug (Schoot DC, Coelingh Bennink HJ, Mannaerts BM, Lamberts SW, Bouchard P, Fauser BC. Human recombinant follicle-stimulating hormone induces growth of preovulatory follicles without concomitant increase in androgen and estrogen biosynthesis in a woman with isolated gonadotropin deficiency. J Clin Endocrinol Metab 1992; 74: 1471-3).
The History of IVF -The Milestones
1991
In Vitro Maturation (IVM) in an unstimulated cycle resulted in pregnancy in a donor oocyte program (Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991 Jan;55(1):109-13).
The fist report suggesting use of the GnRH antagonist, Nal-Glue, to prevent premature LH rise and progesterone in controlled ovarian hyperstimulation treatment. (Frydman R, Cornel C, de Ziegler D, Taieb J, Spitz IM, Bouchard P. Prevention of premature luteinizing hormone and progesterone rise with a gonadotropin-releasing hormone antagonist, Nal-Glu, in controlled ovarian hyperstimulation. Fertil Steril. 1991;56:923-7).
The first report on the use of a laser for Zona Pelucida drilling (Palanker D, Ohad S, Lewis A, Simon A, Shenkar J, Penchas S, Laufer N. Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med. 1991;11(6):580-6).
Navot et all. confirmed that the age-related decline in female fertility is attributable to oocyte quality (Navot D, Bergh PA, Williams MA, Garrisi GJ, Guzman I, Sandler B, Grunfeld L. Poor oocyte quality rather than implantation failure as a cause of age-related decline in female fertility. Lancet. 1991 Jun 8;337(8754):1375-7).
Introduction of GnRH antagonist (Nal-Glu) in IVF procedures (Frydman R, Cornel C, de Ziegler D, Taieb J, Spitz IM, Bouchard P. Prevention of premature luteinizing hormone and progesterone rise with a gonadotropin-releasing hormone antagonist, Nal-Glu, in controlled ovarian hyperstimulation. Fertil Steril. 1991;56(5):923-7).
Embryo transfer catheter is visualized by vaginal ultrasound (Hurley VA, Osborn JC, Leoni MA, Leeton J. Ultrasound-guided embryo transfer: a controlled trial.Fertil Steril. 1991;55(3):559-62).
In Vitro Maturation (IVM) in an unstimulated cycle resulted in pregnancy in a donor oocyte program (Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991 Jan;55(1):109-13).
The fist report suggesting use of the GnRH antagonist, Nal-Glue, to prevent premature LH rise and progesterone in controlled ovarian hyperstimulation treatment. (Frydman R, Cornel C, de Ziegler D, Taieb J, Spitz IM, Bouchard P. Prevention of premature luteinizing hormone and progesterone rise with a gonadotropin-releasing hormone antagonist, Nal-Glu, in controlled ovarian hyperstimulation. Fertil Steril. 1991;56:923-7).
The first report on the use of a laser for Zona Pelucida drilling (Palanker D, Ohad S, Lewis A, Simon A, Shenkar J, Penchas S, Laufer N. Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med. 1991;11(6):580-6).
Navot et all. confirmed that the age-related decline in female fertility is attributable to oocyte quality (Navot D, Bergh PA, Williams MA, Garrisi GJ, Guzman I, Sandler B, Grunfeld L. Poor oocyte quality rather than implantation failure as a cause of age-related decline in female fertility. Lancet. 1991 Jun 8;337(8754):1375-7).
Introduction of GnRH antagonist (Nal-Glu) in IVF procedures (Frydman R, Cornel C, de Ziegler D, Taieb J, Spitz IM, Bouchard P. Prevention of premature luteinizing hormone and progesterone rise with a gonadotropin-releasing hormone antagonist, Nal-Glu, in controlled ovarian hyperstimulation. Fertil Steril. 1991;56(5):923-7).
Embryo transfer catheter is visualized by vaginal ultrasound (Hurley VA, Osborn JC, Leoni MA, Leeton J. Ultrasound-guided embryo transfer: a controlled trial.Fertil Steril. 1991;55(3):559-62).
The History of IVF -The Milestones
1990
The first successful human cleavage-stage embryo vitrification followed by a successful delivery (Gordts S, Roziers P, Campo R, Noto V. Survival and pregnancy outcome after ultrarapid freezing of human embryos. Fertil Steril 1990;53:469-72).
Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. (Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;19;344:768-70).
First report of assisted hatching in human embryos. (Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, Wiemer K. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5(1):7-13).
The first report on polar body biopsy, transfer of the embryo and achieving pregnancy (Verlinsky Y, Ginsberg N, Lifchez A, Valle J, MoiseJ, Strom CM. Analysis of the first polar body: preconception genetic diagnosis. Hum. Reprod. 1990 5: 826-829).
Gonen et al proposed the use of GnRH agonist in place of hCG as a means to trigger the gonadotropic surge for IVF. (Gonen Y, Balakier H, Powell W, Casper RF. Use of gonadotropin-releasing hormone agonist to trigger follicular maturation for in vitro fertilization. J Clin Endocrinol Metab. 1990;71(4):918-22).
Gonen, Jacobson and Casper pioneered the use of combined oral contraceptives for follicle synchronization and cycle scheduling in IVF. (Gonen Y, Jacobson W, Casper RF. Gonadotropin suppression with oral contraceptives before in vitro fertilization. Fertil Steril 1990;53:282-7).
Fishel et all reported about twin birth after subzonal insemination (Fishel S, Antinori S, Jackson P, Johnson J, Lisi F, Chiariello F, Versaci C. Twin birth after subzonal insemination. Lancet. 1990 24;335(8691):722-3).
The first successful human cleavage-stage embryo vitrification followed by a successful delivery (Gordts S, Roziers P, Campo R, Noto V. Survival and pregnancy outcome after ultrarapid freezing of human embryos. Fertil Steril 1990;53:469-72).
Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. (Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;19;344:768-70).
First report of assisted hatching in human embryos. (Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, Wiemer K. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5(1):7-13).
The first report on polar body biopsy, transfer of the embryo and achieving pregnancy (Verlinsky Y, Ginsberg N, Lifchez A, Valle J, MoiseJ, Strom CM. Analysis of the first polar body: preconception genetic diagnosis. Hum. Reprod. 1990 5: 826-829).
Gonen et al proposed the use of GnRH agonist in place of hCG as a means to trigger the gonadotropic surge for IVF. (Gonen Y, Balakier H, Powell W, Casper RF. Use of gonadotropin-releasing hormone agonist to trigger follicular maturation for in vitro fertilization. J Clin Endocrinol Metab. 1990;71(4):918-22).
Gonen, Jacobson and Casper pioneered the use of combined oral contraceptives for follicle synchronization and cycle scheduling in IVF. (Gonen Y, Jacobson W, Casper RF. Gonadotropin suppression with oral contraceptives before in vitro fertilization. Fertil Steril 1990;53:282-7).
Fishel et all reported about twin birth after subzonal insemination (Fishel S, Antinori S, Jackson P, Johnson J, Lisi F, Chiariello F, Versaci C. Twin birth after subzonal insemination. Lancet. 1990 24;335(8691):722-3).
Wednesday, 18 May 2011
Patients Comments - 12
Dear Dr. Silber,
It has been a year this week that a doctor at the University of Chicago told us that we would never have children. As devastating as that news was, we didn't give up. And as luck would have it, God placed into our lives a doctor by the name of Sherman Silber. From the very first day we contacted your office, your staff gave us hope! Something we didn't have much of in the past.
The journey was long and emotional, but nothing worthwhile is ever easy. We are just very happy that the outcome is two wonderful children that we know in our hearts are truly miracles – special gifts from God.
Dr. Silber, I am not sure if you will ever know how much of an impact your work has on the people you serve. But I can tell you this, Gregg and I have not stopped smiling and thanking God since we found out that we were pregnant. You helped give us the best gifts we will ever receive – two children to love and call our own.
Please know that the work you do and the people you help will always be in our prayers. You are a remarkable doctor! Not a day goes by that we don't thank our lucky stars that our paths crossed and we were able to benefit from your tremendous knowledge.
We will continue to keep you updated on the progress of the Kaput twins. So far so good!
Sincerely,
Stephanie & Gregg Kaput
Plainfield, IL
It has been a year this week that a doctor at the University of Chicago told us that we would never have children. As devastating as that news was, we didn't give up. And as luck would have it, God placed into our lives a doctor by the name of Sherman Silber. From the very first day we contacted your office, your staff gave us hope! Something we didn't have much of in the past.
The journey was long and emotional, but nothing worthwhile is ever easy. We are just very happy that the outcome is two wonderful children that we know in our hearts are truly miracles – special gifts from God.
Dr. Silber, I am not sure if you will ever know how much of an impact your work has on the people you serve. But I can tell you this, Gregg and I have not stopped smiling and thanking God since we found out that we were pregnant. You helped give us the best gifts we will ever receive – two children to love and call our own.
Please know that the work you do and the people you help will always be in our prayers. You are a remarkable doctor! Not a day goes by that we don't thank our lucky stars that our paths crossed and we were able to benefit from your tremendous knowledge.
We will continue to keep you updated on the progress of the Kaput twins. So far so good!
Sincerely,
Stephanie & Gregg Kaput
Plainfield, IL
Patients Comments - 11
Dear Dr. Silber,
Thank you very much for helping us conceive our beautiful twin girls, Mallory and Mariah Kortan. They are a wonderful blessing given to us through your help and assistance. We really appreciate what you have done for us. Thanks so very much.
God Bless,
Scott & Tonia Kortan
Palco, KS
Thank you very much for helping us conceive our beautiful twin girls, Mallory and Mariah Kortan. They are a wonderful blessing given to us through your help and assistance. We really appreciate what you have done for us. Thanks so very much.
God Bless,
Scott & Tonia Kortan
Palco, KS
The History of IVF
1989
First report on biopsy of human preimplantation embryos and sexing by DNA amplification. (Handyside AH, Pattinson JK, Penketh RJ, Delhanty JD, Winston RM, Tuddenham EG. Biopsy of human preimplantation embryos and sexing by DNA amplification. Lancet. 1989;18;1:347-9).
First report on the use of laser techniques in the field of assisted reproduction for application in gametes or embryos (Tadir Y, Wright WH, Vafa O, Ord T, Asch RH, Berns MW. Micromanipulation of sperm by a laser generated optical trap. Fertil Steril. 1989 Nov;52(5):870-3).
Gonen and her colleagues in Toronto pioneered the use of ultrasound for endometrial quality (thickness and pattern) related to IVF pregnancy. (Gonen Y, Casper RF, Jacobson W, Blankier J. Endometrial thickness and growth during ovarian stimulation: a possible predictor of implantation in in vitro fertilization. Fertil Steril. 1989;52(3):446-50).
Embryo biopsy technique was developed in mice by Professor Alan Trounson and Leeanda Wilton. (Wilton LJ, Trounson AO. Biopsy of preimplantation mouse embryos: development of micromanipulated embryos and proliferation of single blastomeres in vitro. Biol Reprod. 1989;40(1):145-52).
First report on biopsy of human preimplantation embryos and sexing by DNA amplification. (Handyside AH, Pattinson JK, Penketh RJ, Delhanty JD, Winston RM, Tuddenham EG. Biopsy of human preimplantation embryos and sexing by DNA amplification. Lancet. 1989;18;1:347-9).
First report on the use of laser techniques in the field of assisted reproduction for application in gametes or embryos (Tadir Y, Wright WH, Vafa O, Ord T, Asch RH, Berns MW. Micromanipulation of sperm by a laser generated optical trap. Fertil Steril. 1989 Nov;52(5):870-3).
Gonen and her colleagues in Toronto pioneered the use of ultrasound for endometrial quality (thickness and pattern) related to IVF pregnancy. (Gonen Y, Casper RF, Jacobson W, Blankier J. Endometrial thickness and growth during ovarian stimulation: a possible predictor of implantation in in vitro fertilization. Fertil Steril. 1989;52(3):446-50).
Embryo biopsy technique was developed in mice by Professor Alan Trounson and Leeanda Wilton. (Wilton LJ, Trounson AO. Biopsy of preimplantation mouse embryos: development of micromanipulated embryos and proliferation of single blastomeres in vitro. Biol Reprod. 1989;40(1):145-52).
The History of IVF
1988
First two babies born after epididymal sperm aspiration for men with congenital absence of the vas deferens and naming of the technique MESA (Patrizio P, Silber S, Ord T, Balmaceda JP, Asch RH. Two births after microsurgical sperm aspiration in congenital absence of vas deferens. Lancet. 1988, 10;2(8624):1364).
Australia’s first IVF surrogate birth.
The first baby birth using subzonal sperm injection at National University of Singapore (Ng SC, Bongso A, Ratnam SS, Sathananthan H, Chan CL, Wong PC, Hagglund L, Anandakumar C, Wong YC, Goh VH. Pregnancy after transfer of sperm under zona. Lancet. 1988; 1;2:790).
Pregnancy was obtained from micromanipulation using zona drilling or mechanical partial zona dissection (Cohen J, Malter H, Fehilly C, Wright G, Elsner C, Kort H, Massey J. Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration. Lancet, 1988;16;2:162).
The death of Dr. Patrick Steptoe -March 21 1988. (The Washington Post ,Article: Dr. Patrick Steptoe, 74, Dies; Pioneered Test-Tube Baby Field, March 23, 1988).
The first preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. (Lanzendorf SE, Maloney MK, Veeck LL, Slusser J, Hodgen GD, Rosenwaks Z. A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril. 1988 May;49(5):835-42).
First two babies born after epididymal sperm aspiration for men with congenital absence of the vas deferens and naming of the technique MESA (Patrizio P, Silber S, Ord T, Balmaceda JP, Asch RH. Two births after microsurgical sperm aspiration in congenital absence of vas deferens. Lancet. 1988, 10;2(8624):1364).
Australia’s first IVF surrogate birth.
The first baby birth using subzonal sperm injection at National University of Singapore (Ng SC, Bongso A, Ratnam SS, Sathananthan H, Chan CL, Wong PC, Hagglund L, Anandakumar C, Wong YC, Goh VH. Pregnancy after transfer of sperm under zona. Lancet. 1988; 1;2:790).
Pregnancy was obtained from micromanipulation using zona drilling or mechanical partial zona dissection (Cohen J, Malter H, Fehilly C, Wright G, Elsner C, Kort H, Massey J. Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration. Lancet, 1988;16;2:162).
The death of Dr. Patrick Steptoe -March 21 1988. (The Washington Post ,Article: Dr. Patrick Steptoe, 74, Dies; Pioneered Test-Tube Baby Field, March 23, 1988).
The first preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. (Lanzendorf SE, Maloney MK, Veeck LL, Slusser J, Hodgen GD, Rosenwaks Z. A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril. 1988 May;49(5):835-42).
The History of IVF
1987
Fertilization of human oocytes by microinjection of a single sperm under the zona pellucida (Laws-King A, Trounson A, Sathananthan H, Kola I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril. 1987;48:637-42).
Fertilization of human oocytes by microinjection of a single sperm under the zona pellucida (Laws-King A, Trounson A, Sathananthan H, Kola I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril. 1987;48:637-42).
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